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Cellular GSH synthesis is determined by the availability of the precursor amino acid cysteine as well as the rate-limiting enzyme glutamate cysteine ligase (GCL), which consists of a catalytic (GCLC) and a modifier (GCLM) subunit

This work was supported by the National Natural Science Foundation of China (32402644), the General Program of Liaoning Provincial Science and Technology Joint Fund (2024-MSLH-161) and the Youth Project of the Department of Education of Liaoning Province (jytqn2023439)

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On the other hand, also in the extracellular space, the cysteinyl-glycine fraction is split by the enzyme dipeptidase generating cysteine and glycine